Nanog antibody (AA 1-154)

Catalog No. ABIN306464

The Mouse Monoclonal anti-Nanog antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect Nanog in samples from Human.

Quick Overview for Nanog antibody (AA 1-154) (ABIN306464)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 5A10

Product Details anti-Nanog Antibody

Binding Specificity: AA 1-154

Purification: The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography

Immunogen: Recombinant human Nanog (1-154aa) purified from E. coli

Isotype: IgG2a kappa

Application Details

Application Notes: Recommended dilution 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In Phosphate-Buffered Saline (pH7.4) with 0.1% Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog

Background: Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

NCBI Accession: NP_079141

Pathways: Stem Cell Maintenance