PPP1CA antibody

Catalog No. ABIN306640

This Mouse Monoclonal antibody specifically detects PPP1CA in WB, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PPP1CA antibody (ABIN306640)

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPP1CA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PPP1CA Antibody

Specificity: P16 INK4a

Cross-Reactivity: Human

Cross-Reactivity (Details): Human, other species not tested

Characteristics: pp1a, rabbit anti protein phosphatase 1 alpha,The antibody is suitable to detect p16 in different tissues. Staining results may be cytoplasmatic or nuclear. Nuclear staining should be more specific. Human p16 INK4a (syn. CDKN2, MTS-1) protein is an inhibitor of Cyclin-Dependent-Kinases 4 and 6 (cdk4/cdk6). CDK4 is an important enzyme for the progression of the cell cycle during the G1-Phase. By it's inhibitory action p16 is an important regulator in the cell cycle. The gen encoding for p16 is deleted in many tumour cell lines. In adenocarcinoma of the cervix uteri usually p16 levels are increased. Endocervical p16-overexpression is mostly associated with "high risk" HPV-Typing. In other tumour types as squamous epithelial carcinoma in cervix uteri and head and neck carcinoma p16 expression usually is reduced.

Purification: Protein A affinity purification.

Immunogen: Recombinant human p16 INK4a molecule,Monoclonal antibody DCS-50 is produced after immunisation of Balb/c mice with the full length recombinant p16 INK4a protein of human origin.

Isotype: IgG

Application Details

Application Notes: Pre-treatment with Unmasking Fluid C (Citrate Buffer) or Unmasking Fluid G at 96-100 degree of Celsius is recommended for paraffin sections.,IHC (C,P), IP,60 min at RT,1:10

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: Protein A affinity purified antibody lyophilized from PBS pH 7.4 with BSA and Na-Azide 0.09 % .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for PPP1CA

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Alternative Name: Protein Phosphatase 1 alpha

Pathways: M Phase, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Lipid Metabolism