CAPNL1 antibody

Catalog No. ABIN306693

This anti-CAPNL1 antibody is a Mouse Monoclonal antibody detecting CAPNL1 in WB, IP and EIA. Suitable for Human.

Quick Overview for CAPNL1 antibody (ABIN306693)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAPNL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 15C10

Product Details anti-CAPNL1 Antibody

Cross-Reactivity: Cow, Human, Mouse, Rat

Characteristics: Mouse Anti-Calpain I, u-Calpain (Large subunit, p80), Monoclonal Antibody,The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kD subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (uM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and μcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain I purified from placenta with mouse myeloma.

Isotype: IgG1

Application Details

Application Notes: Use at 0.5-1 μg/mL for ELISA and Western blot, 1-2 μg/mL for immunoprecipitation.

Restrictions: For Research Use only

Handling

Buffer: Provided as a solution containing phosphate buffered saline, 0.08 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

UniProt: P07384

Pathways: Apoptosis