NAPA antibody

Catalog No. ABIN306712

This Mouse Monoclonal antibody specifically detects NAPA in WB, IP and EIA. It exhibits reactivity toward Human.

Quick Overview for NAPA antibody (ABIN306712)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 15D4

Product Details anti-NAPA Antibody

Specificity: Human alpha -SNAP

Cross-Reactivity: Cow, Human, Rat

Characteristics: alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA,SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.

Purification: The antibody was purified to >95% homogeneity by standard chromatographic techniques

Immunogen: Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.,Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP

Isotype: IgG1

Application Details

Application Notes: Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 μg/mL is suggested for western blot and 2-10 μg/mL for immunoprecipitation.

Restrictions: For Research Use only

Handling

Buffer: 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been included in the antibody solution.,100 μg monoclonal antibody in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been added.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: alpha SNAP

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization