LEF-1 Alternate Exon antibody

Catalog No. ABIN306724

This Mouse Monoclonal antibody specifically detects LEF-1 Alternate Exon in WB. It exhibits reactivity toward Human.

Quick Overview for LEF-1 Alternate Exon antibody (ABIN306724)

Target: LEF-1 Alternate Exon

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB)

Clone: 1C3

Product Details

Cross-Reactivity: Human

Characteristics: Mouse Anti Lef-1, Lymphoid Enhancer Factor 1,Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody is specific for an epitope between a.a. 227-231. This sequence lies within the context dependent activation domain of LEF-1 and appears to be an alternate splice variant exon of the LEF-1 gene

Purification: Protein A/G Chromatography

Immunogen: Recombinant full-length LEF-1 protein

Isotype: IgG1

Application Details

Application Notes: Detect LEF-1 protein containing the alternately spliced exon between amino acids 212-241 by Western blot at 1-5 μg/mL. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: LEF-1 Alternate Exon

UniProt: Q9UJU2