PIG3 (p53 Inducible Gene 3) (full length) antibody

Catalog No. ABIN306782

The Rabbit Polyclonal anti-PIG3 (p53 Inducible Gene 3) antibody is suitable to detect PIG3 (p53 Inducible Gene 3) in samples from Human. It has been validated for WB.

Quick Overview for PIG3 (p53 Inducible Gene 3) (full length) antibody (ABIN306782)

Target: PIG3 (p53 Inducible Gene 3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: full length

Purpose: Rabbit anti Human PIG3 (p53 inducible gene 3)

Cross-Reactivity: Human

Characteristics: p53 inducible gene-3 (PIG-3) may be a long-lived reporter, which may be useful for detecting transient activation of p53. The p53 tumor suppressor is the most commonly mutated gene in human cancers. The p53 protein, which is stabilized in response to different biological checkpoints, is activated by DNA damage, hypoxia, viral infection, or oncogene activation resulting in efects such as cell cycle arrest, apoptosis, senescence, differentiation, and antiangiogenesis. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. The p53-inducible gene 3 (PIG3), was recently identified in a screen for genes induced by p53 before the onset of apoptosis. PIG3 shares significant homology with the oxidoreductases from several species. PIG3 protein is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 by p53 occurrs with delayed kinetics as compared with other p53 downstream targets, such as p21 and MDM2. PIG3 levels are increased during p53-mediated growth arrest. Elevated levels of PIG3 are maintained in cells that resume cycling in the absence of ectopic p53 expression.

Purification: Ammonium Sulfate Precipitation

Immunogen: Antibody developed using full-length recombinant PIG-3 protein.

Isotype: IgG

Application Details

Application Notes: Detects recombinant PIG-3 protein by Western blot. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: PIG3 (p53 Inducible Gene 3)

Background: Putative quinone oxidoreductase, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, TP53I3, PIG3