P34 Cdc-2 Nuclear Protein antibody

Catalog No. ABIN306818

The Mouse Monoclonal anti-P34 Cdc-2 Nuclear Protein antibody is suitable to detect P34 Cdc-2 Nuclear Protein in samples from Cow, Human, Mouse, Rat and Xenopus laevis. It has been validated for ICC, IHC (p), IP and WB.

Quick Overview for P34 Cdc-2 Nuclear Protein antibody (ABIN306818)

Target: P34 Cdc-2 Nuclear Protein

Reactivity: Cow, Human, Mouse, Rat, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Clone: POH-1

Product Details

Purpose: Mouse anti Human p34 cdc-2 nuclear protein

Cross-Reactivity: Cow, Human, Mouse, Rat, Xenopus laevis

Characteristics: p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.

Isotype: IgG2a

Application Details

Application Notes: Detects three bands within the 34 kDa region corresponding to p34 and its cleavage products. Can also be used for IHC on paraffin-embedded tissue (10 μg/mL) as well as immunocytochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: P34 Cdc-2 Nuclear Protein

UniProt: P06493