MITF antibody (N-Term)

Catalog No. ABIN306846

This anti-MITF antibody is a Mouse Monoclonal antibody detecting MITF in WB, IHC (p), IP, GS and IHC (fro). Suitable for Human.

Quick Overview for MITF antibody (N-Term) (ABIN306846)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Gel Shift (GS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: C5

Product Details anti-MITF Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: MiTF,There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. MiTF is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteoporotic rat strain harbors a large genomic deletion encompassing the 3 prime half of MiTF including most of the b-HLH-ZIP region. Osteoclasts from these animals lack MiTF protein in contrast to wild-type rat, mouse, and human osteoclasts.

Purification: Protein A/G Chromatography

Immunogen: N-terminal fragment of human microphthalmia protein.,Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.

Isotype: IgG1

Application Details

Application Notes: This antibody can be used for gel supershift assays, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/mL) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, antibody recognizes a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.

Restrictions: For Research Use only

Handling

Buffer: Purified monoclonal antibody clone C5 in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: Microphthalmia Transcription Factor (MiTF)

UniProt: O75030

Pathways: Chromatin Binding