MYOD1 antibody

Catalog No. ABIN306848

The Mouse Monoclonal anti-MYOD1 antibody has been validated for WB, ELISA, IF, IP, IHC (p) and IHC (fro). It is suitable to detect MYOD1 in samples from Human.

Quick Overview for MYOD1 antibody (ABIN306848)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5-2F

Product Details anti-MYOD1 Antibody

Cross-Reactivity: Chicken, Human, Mouse, Pig, Rat

Characteristics: Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3,X1414M monoclonal antibody, clone 5.2F recognizes a protein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue.

Purification: Protein A/G Chromatography

Immunogen: Recombinant mouse MyoD1 protein.,Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

Isotype: IgG2a

Application Details

Application Notes: This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/mL) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 μg/mL). These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer.

Restrictions: For Research Use only

Handling

Buffer: Purified monoclonal antibody 5.2F in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MyoD1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development