Prostaglandin E2 Receptor EP2/EP3 antibody

Catalog No. ABIN306891

The Mouse Monoclonal anti-Prostaglandin E2 Receptor EP2/EP3 antibody (Clone 3F12) (ABIN306891) specifically detects Prostaglandin E2 Receptor EP2/EP3 in WB. The antibody is reactive with Human samples.

Quick Overview for Prostaglandin E2 Receptor EP2/EP3 antibody (ABIN306891)

Target: Prostaglandin E2 Receptor EP2/EP3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB)

Clone: 3F12

Product Details

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Pan Prostaglandin E2 Receptor Subtype EP2 and EP3, Provided under license from Allergan, Inc., United States Patent Pending,Prostaglandins (PG's) are produced by the metabolism of arachidonic acid. PGE-2 is one of the five physiologically significant prostanoids known. Its wide spectrum of physiologic and pharmacologic effects in various tissues are mediated through binding to the PGE-2 receptors (EP1, EP2, EP3 & EP4). These include effects on the immune, endocrine, cardiovascular, renal and reproductive systems as well as smooth muscle. It is also one of the most abundant of the prostanoid family in the brain where it plays an important role in many neural functions, particularly in newborn babies, and as a mediator of inflammation. PGE-2 signals through a family of G-protein coupled receptors known as EP receptors. There are 4 subtypes of EP receptors, known as EP1, EP2, EP3 and EP4. EP2 receptors are 358 amino acid proteins with a short third intracellular loop. EP2 receptors stimulate adenylyl cyclase by their coupling to Gs and do not undergo PGE-2-induced internalization. EP3 receptors are 365-425 amino acid proteins. There are currently 4 known isoforms of EP3 receptors named EP3A, 3B, 3C and 3D. EP3 receptors are involved in water absorption, gastric acid secretion, uterine contraction, neurotransmitter release and the hydrolysis of fat cells (lipolysis) and also act as a mediator of neural inflammation.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human EP2 and EP3 receptor protein and mouse myeloma cells.

Isotype: IgG

Application Details

Application Notes: This antibody can be used for Western blot analysis (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: Prostaglandin E2 Receptor EP2/EP3

Alternative Name: Prostaglandin-E2 receptor EP2/EP3

UniProt: P43116