SNAP25 antibody

Catalog No. ABIN306924

This anti-SNAP25 antibody is a Mouse Monoclonal antibody detecting SNAP25 in WB, ICC and IHC (p). Suitable for Rat.

Quick Overview for SNAP25 antibody (ABIN306924)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: S-7B8

Product Details anti-SNAP25 Antibody

Cross-Reactivity: Chicken, Cow, Human, Monkey, Mouse, Rat

Characteristics: Synaptosome-Associated Protein of 25 kDa,SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells. These two isoforms differ by 9 amino acids in the central portion of the protein.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with rat brain synaptosomal membranes and mouse NS1 cells.

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (1-5 μg/mL), immunocytochemistry and immunohistochemistry on paraformaldehyde fixed CNS tissues (1-5 μg/mL). Does not stain reduced proteins. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport