GAL4 Activation Domain antibody

Catalog No. ABIN307168

This Mouse Monoclonal antibody specifically detects GAL4 Activation Domain in WB and IP. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for GAL4 Activation Domain antibody (ABIN307168)

Target: GAL4 Activation Domain

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 5C8-5

Product Details

Cross-Reactivity: Saccharomyces cerevisiae

Characteristics: GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western Blotting (5-10 μg/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: GAL4 Activation Domain

Alternative Name: GAL-4 Activation Domain