ELAVL1 antibody (AA 1-13)

Catalog No. ABIN307186

This Rabbit Polyclonal antibody specifically detects ELAVL1 in WB, IHC, IP and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig, Chicken, Dog, Sheep, Bat, Horse, Monkey and Rabbit.

Quick Overview for ELAVL1 antibody (AA 1-13) (ABIN307186)

Target: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Chicken, Dog, Sheep, Bat, Horse, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELAVL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ELAVL1 Antibody

Binding Specificity: AA 1-13

Brand: IHC-plus™

Specificity: Recognizes human HuR, Mr ~36kD.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Chicken (100%) Gibbon, Platypus (92%).

Purification: Affinity purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to aa1-13 (MSNGYEDHMAEDC) of human HuR. The immunizing sequence is identical in chicken, mouse, and rat. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Chicken (100%), Gibbon, Platypus (92%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2.0 μg/mL. Detected HuR in RIPA lysates from 3T3/A31 cells. Immunoprecipitation: 4 μg immunoprecipitated HuR from 500 μg of 3T3/A31 RIPA lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in Tris-glycine, 0.15M sodium chloride, 0.05 % sodium azide,  pH 7.4, 30 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ELAVL1

Target: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Alternative Name: ELAVL1 / HUR

Background: Name/Gene ID: ELAVL1

Synonyms: ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG

Gene ID: 1994

UniProt: Q15717

Pathways: AMPK Signaling