Vitamin D-Binding Protein antibody

Catalog No. ABIN307556

The Mouse Monoclonal anti-Vitamin D-Binding Protein antibody has been validated for WB and ELISA. It is suitable to detect Vitamin D-Binding Protein in samples from Human.

Quick Overview for Vitamin D-Binding Protein antibody (ABIN307556)

Target: Vitamin D-Binding Protein (GC)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vitamin D-Binding Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Vitamin D-Binding Protein Antibody

Specificity: Detects human VitD BP in direct ELISAs and Western Blots.

Purification: Protein G purified

Immunogen: Purified hVitD BP from human plasma.

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1-2 μg/mL. Using a colorimetric detection system limit ~2 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL. The detection limit ~10 ng/well. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, VitD BP from human plasma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose

Handling Advice: Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Target Details for Vitamin D-Binding Protein

Target: Vitamin D-Binding Protein (GC)

Alternative Name: GC / Vitamin D-Binding Protein

Background: Name/Gene ID: GC

Synonyms: GC, Gc-globulin, HDBP, Group-specific component, GRD3, VDB, VDBG, VDBP, Vitamin D-binding protein

Gene ID: 2638

UniProt: P02774

Pathways: Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process