DAPP1 antibody (C-Term)

Catalog No. ABIN308258

The Goat Polyclonal anti-DAPP1 antibody has been validated for WB and ELISA. It is suitable to detect DAPP1 in samples from Human.

Quick Overview for DAPP1 antibody (C-Term) (ABIN308258)

Target: DAPP1 (Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DAPP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DAPP1 Antibody

Binding Specificity: C-Term

Purpose: DAPP1

Sequence: SALCISPEEK TDHK

Specificity: This antibody was raised against the C-terminus of NP_055210.1. However, NCBI has changed the version to NP_055210.2 thus losing the C-terminal epitope.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SALCISPEEKTDHK, from the C Terminus of the protein sequence according to AAF14578.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Human Kidney lysates (calculated MW of 32.0 kDa according to AAF14578.1). Recommended concentration: 0.5-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DAPP1

Target: DAPP1 (Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))

Alternative Name: DAPP1

Background: DAPP1, dual adaptor of phosphotyrosine and 3-phosphoinositides, BAM32, DKFZp667E0716, HSPC066, B-cell adapter molecule of 32 kDa, b lymphocyte adapter protein Bam32, dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, hDAPP1

Gene ID: 27071

Pathways: BCR Signaling