IRAK3 antibody (Internal Region)

Catalog No. ABIN308416

This Goat Polyclonal antibody specifically detects IRAK3 in WB and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for IRAK3 antibody (Internal Region) (ABIN308416)

Target: IRAK3 (Interleukin-1 Receptor-Associated Kinase 3 (IRAK3))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IRAK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IRAK3 Antibody

Binding Specificity: Internal Region

Purpose: IRAK3 (mouse)

Sequence: GHSYGSKPME KR

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GHSYGSKPMEKR, from the internal region (near the C Terminus) of the protein sequence according to NP_082955.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65-70 kDa band observed in Rat Liver lysates, and in preliminary testing of Rat Adrenal Gland lysate (calculated MW of 69.0 kDa according to Rat, NP_001101571.1 and 67.8 kDa according to Mouse NP_082955.2). Recommended concentration: 0.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IRAK3

Target: IRAK3 (Interleukin-1 Receptor-Associated Kinase 3 (IRAK3))

Alternative Name: IRAK3

Background: IRAK3, interleukin-1 receptor-associated kinase 3, ASRT5, IRAK-M, IRAKM, interleukin-1 receptor-associated kinase M

Gene ID: 73914, 314870

NCBI Accession: NP_082955

Pathways: TLR Signaling, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades