ABCE1 antibody (N-Term)

Catalog No. ABIN308418

This anti-ABCE1 antibody is a Goat Polyclonal antibody detecting ABCE1 in WB, ELISA, IF and FACS. Suitable for Human.

Quick Overview for ABCE1 antibody (N-Term) (ABIN308418)

Target: ABCE1 (ATP-Binding Cassette, Sub-Family E (OABP), Member 1 (ABCE1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ABCE1 Antibody

Binding Specificity: N-Term

Purpose: ABCE1 / RNase L

Sequence: NHDKCKPKKC RQEC

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence NHDKCKPKKCRQEC, from the N Terminus of the protein sequence according to NP_002931.2, NP_001035809.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Tonsil lysates and in preliminary testing of Human Ovary lysate (calculated MW of 67.3 kDa according to Human NP_002931.2). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperatur

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ABCE1

Target: ABCE1 (ATP-Binding Cassette, Sub-Family E (OABP), Member 1 (ABCE1))

Alternative Name: ABCE1

Background: ABCE1, ATP-binding cassette, sub-family E (OABP), member 1, ABC38, OABP, RLI, RNASEL1, RNASELI, RNS4I, ATP-binding cassette, sub-family E, member 1, RNase L inhibitor, ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) inhibitor

Gene ID: 6059, 24015, 361390

NCBI Accession: NP_002931, NP_001035809