CLTA antibody (Internal Region)

Catalog No. ABIN308426

The Goat Polyclonal anti-CLTA antibody has been validated for WB, IHC and ELISA. It is suitable to detect CLTA in samples from Human.

Quick Overview for CLTA antibody (Internal Region) (ABIN308426)

Target: CLTA (Clathrin, Light Chain A (CLTA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CLTA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CLTA Antibody

Binding Specificity: Internal Region

Purpose: CTLA + CTLB

Sequence: CDFNPKSSKQ AKD

Specificity: This antibody is expected to recognize all reported isoforms of light chains a and b.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CDFNPKSSKQAKD, from the internal region of the protein sequence according to NP_001824.1, NP_009027.1, NP_001070145.1, NP_001825.1, NP_009028.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx. 35 kDa and 22-23 kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2 kDa according to NP_001825.1). The observed molecular weight of ~35 kDa corresponds to earlier findings in literature with different anti

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CLTA

Target: CLTA (Clathrin, Light Chain A (CLTA))

Alternative Name: CLTA

Background: CLTA, CTLB, clathrin, light chain (Lca), LCA, clathrin, light polypeptide (Lca), clathrin, light polypeptide A, clathrin, light chain (Lcb), LCB

Gene ID: 1211, 1212, 12757

NCBI Accession: NP_001824, NP_009027, NP_001070145, NP_001825, NP_009028

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation