HOXD10 antibody (Internal Region)

Catalog No. ABIN308427

This anti-HOXD10 antibody is a Goat Polyclonal antibody detecting HOXD10 in ELISA, IHC and FACS. Suitable for Human.

Quick Overview for HOXD10 antibody (Internal Region) (ABIN308427)

Target: HOXD10 (Homeobox D10 (HOXD10))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HOXD10 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-HOXD10 Antibody

Binding Specificity: Internal Region

Purpose: HOXD10

Sequence: PNRSCRIEQP VTQQ

Cross-Reactivity: Cow, Horse, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PNRSCRIEQPVTQQ, from the internal region of the protein sequence according to NP_002139.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in some epithelial cells of PCT. Recommended concentration, 3-5 μg/mL.

Western Blot: Preliminary testing showed a band at approx 37 kDa in Human Kidney lysate after 3 μg/mL antibody staining (calculated MW of 38.4 kDa according to NP_002139.2). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HOXD10

Target: HOXD10 (Homeobox D10 (HOXD10))

Alternative Name: HOXD10

Background: HOXD10, homeobox D10, HOX4, HOX4D, HOX4E, Hox-4.4, homeo box 4D, homeo box D10

Gene ID: 3236, 15430, 303991

NCBI Accession: NP_002139