ERP29 antibody (C-Term)

Catalog No. ABIN308450

The Goat Polyclonal anti-ERP29 antibody has been validated for WB, ELISA and IF. It is suitable to detect ERP29 in samples from Human and Rat.

Quick Overview for ERP29 antibody (C-Term) (ABIN308450)

Target: ERP29 (Endoplasmic Reticulum Protein 29 (ERP29))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ERP29 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ERP29 Antibody

Binding Specificity: C-Term

Purpose: ERP29

Sequence: EKNKMSDGKK EELQ

Specificity: This antibody is expected to recognize isoform 1 (NP_006808.1) only.

Cross-Reactivity: Cow, Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKNKMSDGKKEELQ, from the C Terminus of the protein sequence according to NP_006808.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 28 kDa band observed in Human Liver lysates (calculated MW of 29.0 kDa according to NP_006808.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This product has been successfully used in WB on Rat: Gao J et a

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Immunofluorescence:This product has been successfully used in IF on Rat: Gao J et al, Mol Med Rep. 2016 Jan,13(1):398-402. PMID: 26548430.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ERP29

Target: ERP29 (Endoplasmic Reticulum Protein 29 (ERP29))

Alternative Name: ERP29

Background: ERP29, endoplasmic reticulum protein 29, C12orf8, ERp28, ERp31, PDI-DB, endoplasmic reticulum lumenal protein ERp28

Gene ID: 10961, 67397, 117030

NCBI Accession: NP_006808