IDS antibody (Internal Region)

Catalog No. ABIN308452

This Goat Polyclonal antibody specifically detects IDS in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for IDS antibody (Internal Region) (ABIN308452)

Target: IDS (Iduronate 2-Sulfatase (IDS))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IDS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-IDS Antibody

Binding Specificity: Internal Region

Purpose: IDS

Sequence: KHFRFRDLEE DP

Specificity: This antibody is expected to recognize isoform a (NP_000193.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows lysosomal staining of trophoblasts. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx. 60 kDa band observed in Human Liver lysates (calculated MW of 61.9 kDa according to NP_000193.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDS

Target: IDS (Iduronate 2-Sulfatase (IDS))

Alternative Name: IDS

Background: IDS, iduronate 2-sulfatase (Hunter syndrome), MPS2, SIDS, iduronate-2-sulfatase

Gene ID: 3423

NCBI Accession: NP_000193

Pathways: Glycosaminoglycan Metabolic Process