AUTS2 antibody (C-Term)

Catalog No. ABIN308459

The Goat Polyclonal anti-AUTS2 antibody has been validated for ELISA, IF and FACS. It is suitable to detect AUTS2 in samples from Human. There is 1 publication available.

Quick Overview for AUTS2 antibody (C-Term) (ABIN308459)

Target: AUTS2 (Autism Susceptibility Candidate 2 (AUTS2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AUTS2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-AUTS2 Antibody

Binding Specificity: C-Term

Purpose: AUTS2

Sequence: ERPPSHTLKD IEAR

Specificity: This antibody is expected to recognize NP_056385.1 isoforms 1 and NP_001120703.1 isoform 2.

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ERPPSHTLKDIEAR , from the C Terminus of the protein sequence according to NP_056385.1, NP_001120703.1.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-AUTS2 antibody (ABIN308459)

Oksenberg, Haliburton, Eckalbar, Oren, Nishizaki, Murphy, Pollard, Birnbaum, Ahituv: "Genome-wide distribution of Auts2 binding localizes with active neurodevelopmental genes." in: Translational psychiatry, Vol. 4, pp. e431, (2014) (PubMed).

Target Details for AUTS2

Target: AUTS2 (Autism Susceptibility Candidate 2 (AUTS2))

Alternative Name: AUTS2

Background: AUTS2, autism susceptibility candidate 2, KIAA0442, MGC13140, autism-related protein 1

Gene ID: 26053, 319974

NCBI Accession: NP_056385, NP_001120703