BAG4 antibody (Internal Region)

Catalog No. ABIN308469

This anti-BAG4 antibody is a Goat Polyclonal antibody detecting BAG4 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for BAG4 antibody (Internal Region) (ABIN308469)

Target: BAG4 (BCL2-Associated Athanogene 4 (BAG4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAG4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-BAG4 Antibody

Binding Specificity: Internal Region

Purpose: SODD

Sequence: GNATSDHPNN QDQSS

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GNATSDHPNNQDQSS, from the internal region of the protein sequence according to NP_004865.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx. 60 kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6 kDa according to NP_004865.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BAG4

Target: BAG4 (BCL2-Associated Athanogene 4 (BAG4))

Alternative Name: BAG4

Background: BAG4, BCL2-associated athanogene 4, BAG-4, SODD, BAG-family molecular chaperone regulator-4, silencer of death domains

Gene ID: 9530

NCBI Accession: NP_004865

Pathways: Regulation of Actin Filament Polymerization