STN8 Kinase (AA 425-438) antibody

Catalog No. ABIN3155736

This Rabbit Polyclonal antibody specifically detects STN8 Kinase in WB. It exhibits reactivity toward Arabidopsis thaliana and Triticum aestivum.It has been mentioned in 1 publication

Quick Overview for STN8 Kinase (AA 425-438) antibody (ABIN3155736)

Target: STN8 Kinase

Reactivity: Arabidopsis thaliana, Triticum aestivum

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: AA 425-438

Purpose: Anti-STN8 | Serine/threonine-protein kinase STN8 (chloroplastic)

Cross-Reactivity (Details): Arabidopsis thaliana, Triticum aestivum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Oryza sativa

Purification: Immunogen affinity purified serum

Immunogen: KLH-conugated synthetic peptide (amino acids 425-438) specific for Arabidopsis thaliana STN8 serine/threonine proteinkinase

Application Details

Application Notes: 1 : 2000 (WB)

Comment:

For best results with this antibody sample buffer needs to contain 6 M urea (138mM TrisHCl pH 6.8, 6M urea, 22.2% Glycerol, 4.3% SDS).STN8 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn8 is 49 amino acids. STN8 portein mobility can be also affected by urea present in the gel.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Woeber: "Relationship between thyroid stimulating hormone and thyroid stimulating immunoglobulin in Graves' hyperthyroidism." in: Journal of endocrinological investigation, Vol. 34, Issue 3, pp. 222-4, (2011) (PubMed).

Target Details

Target: STN8 Kinase

Background:

Background: Serine/threonine protein kinase (EC=2.7.11.1), known as STN8 in Arabidopsis, and its Stl1 homologue in Chlamydomonas, are STN7/Stt7-like proteins that are not required for state transitions. The Arabidopsis mutants deficient in stn8 gene demonstrated highly reduced levels of the phosphorylated proteins of the photosystem II (Bonardi et al., 2005 Varionen et al., 2005). Alternative name: Protein STATE TRANSITION 8.

Additional information: An extract from STN8 mutant needs to be used in pararel to determine specific band of STN8 protein on a western blot

Molecular Weight: 54.9 kDa or 46 kDa on 6 M urea gel

Gene ID: 831741

UniProt: Q9LZV4