CD44 antibody

Catalog No. ABIN180800

The Mouse Monoclonal anti-CD44 antibody has been validated for FACS, IHC (p), IP and IHC (fro). It is suitable to detect CD44 in samples from Human.

Quick Overview for CD44 antibody (ABIN180800)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Bu52

Product Details anti-CD44 Antibody

Specificity: This antibody recognises the CD44 cell surface antigen, a 100 kDa glycoprotein.

Characteristics: Synonyms: CDw44, PGP-I, ECMR-III, Hermes antigen, Epican, LHR, MDU2, MDU3, MIC4, Phagocyticglycoprotein I, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesionreceptor, Hyaluronate receptor, Heparan sulfate proteoglycan

Purification: Affinity Chromatography on Protein G

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/25-1/100 diuted antibody to label 1x10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/100-1/200 (Requires antigen retrieval usingheat treatment with citrate buffer pH 6.0 prior to staining of paraffin sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44 is widely expressed on human leucocytes,white matter of the brain and by some epithelial cells of the intestine and of the breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. The Bu52 antibody recognises an epitope that is common to all CD44 isoforms. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling