POMC antibody (N-Term)

Catalog No. ABIN316639

This Mouse Monoclonal antibody specifically detects POMC in IHC (p) and EIA. It exhibits reactivity toward Human and Rat.

Quick Overview for POMC antibody (N-Term) (ABIN316639)

Target: POMC (Proopiomelanocortin (POMC))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This POMC antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 57 (BGN-1388-66)

Product Details anti-POMC Antibody

Binding Specificity: AA 1-24, N-Term

Specificity: This ACTH antibody (Clone 57, BGN/1388/66) recognises 1-24 ACTH (Synacthen) which, as a synthetic analogue of naturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserve and for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation. It does not recognise 17-39 ACTH (CLIP). Cross-reacts with Rat N-terminal ACTH.

Characteristics: Synonyms: Corticotropin, Adrenocorticotropic hormone, ACTH

Purification: Purified IgG prepared by affinity chromatography on Protein A

Immunogen: Synthetic peptide corresponding to the amino acids 1-24 of the Human ACTH conjugated toKeyhole Limpet Haemocyanin. AA Sequence: SYSMEHFRWGKPVGKKRRPVKVYP

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunohistochemistry on Formalin Fixed Paraffin Embedded Sections: 1/100-1/200(Requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for POMC

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: POMC

Background: ACTH, released from the anterior pituitary gland in response to corticotropin-releasing hormone from the hypothalamus, acts on the adrenal cortex to stimulate the production of corticosteroids such as cortisol, involved in the response to stress. Studies have shown that the administration of 1-24 ACTH increases blood pressure, believed to be attributed to an increase in ACTH-stimulated cortisol secretion, in association with increased cardiac output.Synonyms: Adrenocorticotropic hormone, Corticotropin, POMC

Gene ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour