Granulocytes antibody (FITC)

Catalog No. ABIN317010

The FITC-conjugated Mouse Monoclonal anti-Granulocytes antibody (Clone 2B2) (ABIN317010) specifically detects Granulocytes in FACS. The antibody is reactive with Pig samples.

Quick Overview for Granulocytes antibody (FITC) (ABIN317010)

Target: Granulocytes

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS)

Clone: 2B2

Product Details

Cross-Reactivity (Details): Species reactivity (tested):Pig.

Characteristics: Synonyms: Granulocyte marker

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Porcine bone marrow haematopoietic cells (BMHC).

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 1x10^6 cells in 100 μL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Granulocytes

Background: Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules, Eosinophils have prominent granules that stain readily with the acid dye eosin, and Basophils have prominent granules that stain readily basic (non acidic) dyes.Synonyms: Granulocyte cells