C3 antibody

Catalog No. ABIN317012

The Mouse Monoclonal anti-C3 antibody has been validated for WB, IHC (fro), FACS and EIA. It is suitable to detect C3 in samples from Human.

Quick Overview for C3 antibody (ABIN317012)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: 013III-1-16

Product Details anti-C3 Antibody

Specificity: AM05608PU-N Recognises 42 kDa inactive complement component 3b (iC3b) neoantigen present in blood serum.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: CPAMD1, Complement component 3, C3 and PZP-like alpha-2-macroglobulindomain-containing protein 1

Purification: Purified IgG prepared by affinity chromatography on Protein A

Immunogen: Purified Human iC3b

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot. Flow cytometry. Immunohistochemistry on Frozen Sections. Histology Positive Control Tissue: Kidney from post streptoccal glomerulonephritispatients.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Borate buffered saline pH 8.2-8.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3

Background: Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

Gene ID: 718

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling