IL-4 antibody (PE)

Catalog No. ABIN317378

The Mouse Monoclonal anti-IL-4 antibody has been validated for FACS. It is suitable to detect IL-4 in samples from Dog, Cow, Pig, Sheep, Goat and Mustelid.

Quick Overview for IL-4 antibody (PE) (ABIN317378)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Dog, Cow, Pig, Sheep, Goat, Mustelid

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: CC303

Product Details anti-IL-4 Antibody (PE)

Specificity: This antibody recognises bovine interleukin 4.

Characteristics: Synonyms: IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra, B-cell stimulatory factor1

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1/5.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 1 mL distilled water

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide, 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: Interleukin-4 / IL4

Background: IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra

Gene ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation