CD52 antibody

Catalog No. ABIN317437

This Rat Monoclonal antibody specifically detects CD52 in FACS, WB, IHC (fro) and CyTox. It exhibits reactivity toward Human and Monkey.

Quick Overview for CD52 antibody (ABIN317437)

Target: CD52

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This CD52 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Clone: YTH66-9HL

Product Details anti-CD52 Antibody

Specificity: This antibody reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29 kDa.

Characteristics: Synonyms: HE5, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5

Purification: Ammonium sulphate precipitation

Immunogen: Human T lymphocytes

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1: 20. Western blot. Flow cytometry: use 10 μL of 1: 20 diluted antibody to label 10^6 cells in 100 μL. Cytotoxic assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer: Phosphate buffered saline 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Should this product contain a precipitate we recommendmicrocentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD52

Target: CD52

Alternative Name: CD52 / CAMPATH-1

Background: CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is epressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

Gene ID: 1043

UniProt: P31358