CD52 antibody

Catalog No. ABIN317437

Product Details anti-CD52 Antibody

Target: CD52

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This CD52 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Specificity: This antibody reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29 kDa.

Characteristics: Synonyms: HE5, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5

Purification: Ammonium sulphate precipitation

Immunogen: Human T lymphocytes

Clone: YTH66-9HL

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1: 20. Western blot. Flow cytometry: use 10 μL of 1: 20 diluted antibody to label 10^6 cells in 100 μL. Cytotoxic assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer: Phosphate buffered saline 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Should this product contain a precipitate we recommendmicrocentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD52

Target: CD52

Alternative Name: CD52 / CAMPATH-1 (CD52 Products)

Background: CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is epressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

Gene ID: 1043

UniProt: P31358