CD8 antibody (PE)

Catalog No. ABIN317442

This anti-CD8 antibody is a Mouse Monoclonal antibody detecting CD8 in FACS. Suitable for Sheep, Cow and Goat.

Quick Overview for CD8 antibody (PE) (ABIN317442)

Target: CD8

Reactivity: Sheep, Cow, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: CC63

Product Details anti-CD8 Antibody (PE)

Specificity: This antibody reacts with the CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of 34 kDa and 34 kDa under reducing conditions.

Characteristics: Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 1 mL distilled water.

Buffer: Phosphate buffered saline 0.09 % Sodium Azide, 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE. This product isphotosensitive and should be protected from light. Should this product contain aprecipitate we recommend microcentrifugation before use.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 281060

UniProt: P31783