STEAP1 antibody (N-Term)

Catalog No. ABIN317568

The Mouse Monoclonal anti-STEAP1 antibody (Clone J2D2) (ABIN317568) specifically detects STEAP1 in WB, IF, IHC (p) and EIA. The antibody is reactive with Human and Rat samples.

Quick Overview for STEAP1 antibody (N-Term) (ABIN317568)

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This STEAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: J2D2

Product Details anti-STEAP1 Antibody

Binding Specificity: AA 1-70, N-Term

Specificity: The antibody recognizes STEAP1.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Characteristics: Synonyms: STEAP-1, Metalloreductase STEAP1, PRSS24, Six-transmembrane epithelial antigen ofprostate 1

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human STEAP1 (1-70 aa) purified from E. coli

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot (cell lysates of LNcap (30 μg) and Rat testis (30 μg) were resolved bySDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1: 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPand an ECL detection system). Immunofluorescence (1: 100 - 1: 300). Immunohistochemsitry on paraffin sections (1: 50 - 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for STEAP1

Target: STEAP1 (Six Transmembrane Epithelial Antigen of The Prostate 1 (STEAP1))

Alternative Name: STEAP / STEAP1

Background: Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy. NCBI Accession No: NP_036581Synonyms: Metalloreductase STEAP1, PRSS24, STEAP-1, Six-transmembrane epithelial antigen of prostate 1

Gene ID: 26872

UniProt: Q9UHE8

Pathways: Transition Metal Ion Homeostasis