S1PR2 antibody

Catalog No. ABIN317633

Product Details anti-S1PR2 Antibody

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of EDG-5 protein.(region surrounding Leu303)

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-S1PR2 antibody (ABIN317633)

Li, Fotinos, Wu, Chen, Zhu, Baranov, Tu, Zhou, Sinha, Kristal, Wang: "N-acetyl-L-tryptophan delays disease onset and extends survival in an amyotrophic lateral sclerosis transgenic mouse model." in: Neurobiology of disease, Vol. 80, pp. 93-103, (2015) (PubMed).

Adler, Leich, Ellinger-Ziegelbauer, Hewitt, Dekant, Rosenwald, Mally: "Application of RNA interference to improve mechanistic understanding of omics responses to a hepatotoxic drug in primary rat hepatocytes." in: Toxicology, Vol. 326, pp. 86-95, (2015) (PubMed).

Wang, Miao, Li, Yao, Sun, Li, Zhao, Guo: "CXCL12/CXCR4 axis confers adriamycin resistance to human chronic myelogenous leukemia and oroxylin A improves the sensitivity of K562/ADM cells." in: Biochemical pharmacology, Vol. 90, Issue 3, pp. 212-25, (2014) (PubMed).

Cruz-Orengo, Daniels, Dorsey, Basak, Grajales-Reyes, McCandless, Piccio, Schmidt, Cross, Crosby, Klein: "Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility." in: The Journal of clinical investigation, Vol. 124, Issue 6, pp. 2571-84, (2014) (PubMed).

Huang, Liu, Lan, Xie, Peng, Huang, Wang, Shen, Liu, Huang: "Berberine reduces fibronectin expression by suppressing the S1P-S1P2 receptor pathway in experimental diabetic nephropathy models." in: PLoS ONE, Vol. 7, Issue 8, pp. e43874, (2012) (PubMed).

Danieli-Betto, Peron, Germinario, Zanin, Sorci, Franzoso, Sandonà, Betto: "Sphingosine 1-phosphate signaling is involved in skeletal muscle regeneration." in: American journal of physiology. Cell physiology, Vol. 298, Issue 3, pp. C550-8, (2010) (PubMed).

Target Details for S1PR2

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Alternative Name: EDG-5 / S1P2 (S1PR2 Products)

Background: The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high-affinity. EDG-6 is a low-affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain. EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.Synonyms: AGR16, EDG5, Endothelial differentiation G-protein coupled receptor 5, Gpcr13, H218, LPB2, S1P receptor 2, S1PR2, Sphingosine 1-phosphate receptor 2

Molecular Weight: approx. 39 kDa

Gene ID: 9294

NCBI Accession: NP_004221

UniProt: O95136

Pathways: Synaptic Membrane