CCKAR antibody

Catalog No. ABIN317645

The Rabbit Polyclonal anti-CCKAR antibody has been validated for WB, IF and EIA. It is suitable to detect CCKAR in samples from Human, Rat and Mouse.

Quick Overview for CCKAR antibody (ABIN317645)

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCKAR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-CCKAR Antibody

Specificity: This antibody detects endogenous levels of CCK-AR protein.(region surrounding Pro255)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CCKAR

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Alternative Name: CCK-A Receptor

Background: The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous important physiologic events. These appear to be mediated through 2 general classes of receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family peptides. Through binding to class A receptors, CCK is a major physiologic mediator of gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion. Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the central nervous system, where they have been implicated in the pathogenesis of feeding disorders, Parkinson disease, schizophrenia, and drug addiction.Synonyms: CCKA Receptor, CCKAR, CCKRA, Cholecystokinin receptor type A, Cholecystokinin-1 receptor

Gene ID: 886

NCBI Accession: NP_000721

UniProt: P32238

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour