EDG4 antibody

Catalog No. ABIN317689

This Rabbit Polyclonal antibody specifically detects EDG4 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for EDG4 antibody (ABIN317689)

Target: EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDG4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-EDG4 Antibody

Specificity: This antibody detects endogenous levels of EDG-4 protein (region surrounding Gln330).

Purification: Affinity-chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EDG4

Target: EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))

Alternative Name: EDG-4 / LPAR2

Background: The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high-affinity. EDG-6 is a low-affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.Synonyms: EDG4, Ipa-2, LPAR2, Lysophosphatidic acid receptor 2, Lysophosphatidic acid receptor Edg-4, endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor 4

Molecular Weight: approx. 39 kDa

Gene ID: 9170

NCBI Accession: NP_004711

UniProt: Q9HBW0