IP6K2 antibody

Catalog No. ABIN317720

The Rabbit Polyclonal anti-IP6K2 antibody has been validated for WB, IF, IHC (p) and EIA. It is suitable to detect IP6K2 in samples from Human, Mouse and Rat.

Quick Overview for IP6K2 antibody (ABIN317720)

Target: IP6K2 (Inositol Hexakisphosphate Kinase 2 (IP6K2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IP6K2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-IP6K2 Antibody

Specificity: This antibody detects endogenous levels of InsP6 kinase 2 / IP6K2 protein (region surrounding Leu188).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 15 mM Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IP6K2

Target: IP6K2 (Inositol Hexakisphosphate Kinase 2 (IP6K2))

Alternative Name: InsP6 Kinase 2 / IP6K2

Background: The members of the inositol hexakisphosphate kinase family, IP6K1 and IP6K2, have a high affinity and selectivity for inositol hexakisphosphate (InsP6) as a substrate. IP6K1 and IP6K2 (also designated PiUS) convert InsP6 to PP-InsP5. However, neither kinase demonstrates any catalytic activity with other inositol pyrophosphates. The presence of InsP6, which inhibits serine/threonine protein phosphatases, increases the influx of calcium across the plasma membrane and implies that it may mediate the regulation of insulin exocytosis. IP6K1 was purified as a 54 kDa protein in rat brain extracts. By homology, IP6K1 and IP6K2 were characterized in mouse as a 50 kDa and 49 kDa protein, respectively. IP6K1 displays ATP synthase activity by transferring a phosphate from PP-InsP5 to ADP, which suggests a role for the IP6 kinases as high energy phosphate donors.Synonyms: IHPK2, Inositol hexakisphosphate kinase 2, P(i)-uptake stimulator, PiUS, TCCCIA00113

Molecular Weight: approx. 52 kDa

Gene ID: 51447

NCBI Accession: NP_001005909

UniProt: Q9UHH9