APOL1 antibody

Catalog No. ABIN317817

The Rabbit Polyclonal anti-APOL1 antibody (ABIN317817) specifically detects APOL1 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for APOL1 antibody (ABIN317817)

Target: APOL1 (Apolipoprotein L, 1 (APOL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOL1 Antibody

Specificity: ApoL1 antibody detects endogenous levels of ApoL1 protein.(region surrounding Gln294)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOL1

Target: APOL1 (Apolipoprotein L, 1 (APOL1))

Alternative Name: Apolipoprotein L1 (Apo L1)

Background: Apolipoproteins are protein components of plasma lipoproteins. The apolipoprotein L gene family encodes six highly homologous proteins designated apoL-I to -VI, which are associated with large high density type lipoproteins (HDL). The human apoL family maps to chromosome 22q12.1- 13.1 within a 127,000-bp region . apoL has been characterized as a pancreas specific, 383-amino acid, 42 kDa protein that contains a 12-amino acid secretory signal peptide. The apoL genes have TATA-less promoters and contain putative sterol regulatory elements, suggesting that transcription of these genes may be coordinated with that of the low density lipoprotein receptor and genes in pathways involving the synthesis of triglycerides and cholesterol. apoL homologs can undergo 10 fold changes in expression during atherosclerotic changes in vascular endothelial cells, which includes the inflammatory reaction of atherosclerotic lesions.Synonyms: APOL1, ApoL-I, Apolipoprotein L-I

Molecular Weight: approx. 44 kDa

Gene ID: 8542

NCBI Accession: NP_001130012

UniProt: O14791