CA5B antibody

Catalog No. ABIN317824

This Rabbit Polyclonal antibody specifically detects CA5B in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CA5B antibody (ABIN317824)

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA5B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CA5B Antibody

Specificity: This antibody detects endogenous levels of CA VB protein.(region surrounding asn280)

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CA5B

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Alternative Name: Carbonic Anhydrase 5B

Background: Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes responsible for catalyzing the reversible hydration of carbon dioxide.CAs show extensive diversity in their distribution and subcellular localization.They are involved in a variety of biological processes, including calcification, bone resorption, respiration, acid-base balance and the formation of aqueous humor, saliva, gastric juice and cerebrospinal fluid. CA VB, also known as carbonate dehydratase VB, is one of two isoforms of CA V. It localizes to the mitochondria and is involved in metabolic processes. CA VB is predominantly expressed in heart, pancreas, lung, placenta, kidney and skeletal muscle. It exhibits highest homology with family member CA VA (the second isoform of CA V), however, unlike CA VA, it is not expressed in the liver, suggesting that it plays a significantly different physiological role.Synonyms: CA-VB, CA5B, Carbonate dehydratase VB, Carbonic anhydrase VB

Molecular Weight: approx. 38 kDa

Gene ID: 11238

NCBI Accession: NP_009151

UniProt: Q9Y2D0