CLCN4 antibody

Catalog No. ABIN317826

The Rabbit Polyclonal anti-CLCN4 antibody (ABIN317826) specifically detects CLCN4 in WB and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CLCN4 antibody (ABIN317826)

Target: CLCN4 (Chloride Channel 4 (CLCN4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLCN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-CLCN4 Antibody

Specificity: This antibody detects endogenous levels of CLC-4 protein.(region surrounding Glu254)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 60000approx. 1: 80000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CLCN4

Target: CLCN4 (Chloride Channel 4 (CLCN4))

Alternative Name: CLCN4

Background: The family of voltage-dependent chloride channels (CLCs) regulate cellular trafficking of chloride ions, a critical component of all living cells. CLCs regulate excitability in muscle and nerve cells, aid in organic solute transport and maintain cellular volume. The genes encoding human CLC-1 through CLC-7 map to chromosomes 7, 3q26, 4q32, Xp22, Xp11, 1p36 and 16p13, respectively. CLC-1 is highly expressed in skeletal muscle. Mutations in the gene encoding CLC-1 lead to myotonia, an inheritable disorder characterized by muscle stiffness and renal salt wasting. CLC-2 is highly expressed in the epithelia of several organs including lung, which suggests CLC-2 may be a possible therapeutic target for cystic fibrosis. CLC-3 expression is particularly abundant in neuronal tissue, while CLC-4 expression is evident in skeletal and cardiac muscle as well as brain. Mutations in the gene encoding CLC-5 lead to Dent's disease, a renal disorder characterized by proteinuria and hypercalciuria. CLC-6 and CLC-7 are broadly expressed in several tissues including testes, kidney, brain and muscle.Synonyms: CLC4, Chloride channel protein 4, Chloride transporter ClC-4, ClC-4, ClC4, H(+)/Cl(-) exchange transporter 4

Molecular Weight: approx. 85 kDa

Gene ID: 1183

NCBI Accession: NP_001821

UniProt: P51793