ALDOB antibody

Catalog No. ABIN317837

The Rabbit Polyclonal anti-ALDOB antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect ALDOB in samples from Human, Rat and Mouse. There is 1 publication available.

Quick Overview for ALDOB antibody (ABIN317837)

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDOB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ALDOB Antibody

Specificity: This antibody detects endogenous levels of Aldolase B protein.(region surrounding Trp148)

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 with 0.05 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ALDOB antibody (ABIN317837)

Walmsley, Freund, Curthoys: "Proteomic profiling of the effect of metabolic acidosis on the apical membrane of the proximal convoluted tubule." in: American journal of physiology. Renal physiology, Vol. 302, Issue 11, pp. F1465-77, (2012) (PubMed).

Target Details for ALDOB

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Alternative Name: Aldolase B / ALDOB

Background: Fructose 1,6-bisphosphate Aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate.Fructose 1,6-bisphosphate Aldolase exists as three forms, the muscle-specific Aldolase A, the liver-specific Aldolase B, and the brain-specific Aldolase C. Aldolase A, B, and C arose from a common ancestral gene, from which Aldolase B first diverged. Aldolase A is one of the most highly conserved enzymes known, with only about 2 % of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagons and has been implicated in hereditary fructose intolerance disease. Aldolase C is 36 kDa polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells.Synonyms: Fructose-bisphosphate aldolase B, Liver-type aldolase

Molecular Weight: approx. 45 kDa

Gene ID: 229

NCBI Accession: NP_000026

UniProt: P05062