NHE7 antibody

Catalog No. ABIN317937

The Rabbit Polyclonal anti-NHE7 antibody (ABIN317937) specifically detects NHE7 in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for NHE7 antibody (ABIN317937)

Target: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHE7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NHE7 Antibody

Specificity: NHE-7 antibody detects endogenous levels of NHE-7 protein.(region surrounding Asp566)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NHE7

Target: NHE7 (SLC9A7) (Sodium Hydrogen Exchanger 7 (SLC9A7))

Alternative Name: SLC9A7 / NHE7

Background: Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH ( pH i) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. NHE7 (human 725 aa, chromosome Xp11.4) is ubiquitously expressed, and predominantly localizes to the trans-golgi network. NHE7 mediates the influx of Na+ or K+ in exchange for H+. It is ~70 % related to NHE6 but relatively less (~25 %) homologous with other NHEs.Synonyms: NHE-7, Na(+)/H(+) exchanger 7, Sodium/hydrogen exchanger 7, Solute carrier family 9 member 7

Molecular Weight: approx. 80 kDa

Gene ID: 84679

NCBI Accession: NP_115980

UniProt: Q96T83