SMAD3 antibody (pSer204)
This Rabbit Polyclonal antibody specifically detects SMAD3 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.
Quick Overview for SMAD3 antibody (pSer204) (ABIN6281329)
Target
See all SMAD3 Antibodies
SMAD3
(SMAD, Mothers Against DPP Homolog 3 (SMAD3))
Reactivity
Human, Mouse, Rat
Host
Rabbit
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
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Binding Specificity
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pSer204
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Specificity
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Phospho-Smad3 (S204) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S204.
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Characteristics
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Rabbit Polyclonal to Phospho-Smad3 (S204).
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Immunogen
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Synthesized peptide derived from human Smad3 around the phosphorylation site of S204.
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Isotype
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IgG
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Application Notes
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WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000,
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Restrictions
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For Research Use only
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Format
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Liquid
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Concentration
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1 mg/mL
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Buffer
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Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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Preservative
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Sodium azide
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Precaution of Use
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This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
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Avoid repeated freeze/thaw cycles.
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Storage
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-20 °C
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Storage Comment
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Store at -20°C.
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Target
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SMAD3
(SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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Alternative Name
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Smad3
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Molecular Weight
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48 kDa
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Gene ID
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4088
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UniProt
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P84022
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Pathways
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Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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