PDCD4 antibody (C-Term)

Catalog No. ABIN318336

The Rabbit Polyclonal anti-PDCD4 antibody (ABIN318336) specifically detects PDCD4 in WB, IHC (p), EIA and IHC (fro). The antibody is reactive with Human, Mouse, Rat, Xenopus laevis and Staphylococcus aureus samples.

Quick Overview for PDCD4 antibody (C-Term) (ABIN318336)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human, Mouse, Rat, Xenopus laevis, Staphylococcus aureus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PDCD4 Antibody

Binding Specificity: C-Term

Specificity: The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.

Cross-Reactivity (Details): Species reactivity (expected):A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from Human, Mouse, Rat and Xenopus based on 100 % homology with the immunizing sequence.
Species reactivity (tested):Human, Mouse, Rat, S. aureus, Xenopus.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding amino acids near the carboxyl terminus of Human Pdcd4 protein.

Application Details

Application Notes: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/10,000 (a band approximately 52 kDa in size corresponding toPdcd4 protein in the appropriate cell lysate or extract). Immunohistochemistry: 1: 100-1/300.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.06 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4

Background: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gene ID: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6