BMI1 antibody (Middle Region)

Catalog No. ABIN318769

This Rabbit Polyclonal antibody specifically detects BMI1 in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BMI1 antibody (Middle Region) (ABIN318769)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-BMI1 Antibody

Binding Specificity: Middle Region

Specificity: This antibody recognises 37 kDa polycomb complex protein BMI-1, involved in segment specification, cell growth and maintenance, transcriptional regulation and chromatin modification.

Purification: Purified

Immunogen: 15 amino acid peptide sequence near the centre of human BMI-1.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 - 1.0g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI-1 / RNF51

Background: BMI-1 is a proto-oncogene which cooperates with the myc oncogene to produce B-lymphomas. It is required in the regulation of normal adult and leukaemic stem cells, making BMI-1 a potential target for stem cell proliferation and anti-cancer therapies. BMI-1 represses transcription at the ink4 locus which contains tumour suppressors p16 and p19Arf. BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex.Synonyms: BMI1, PCGF4, Polycomb group RING finger protein 4, RING finger protein 51

Gene ID: 100532731

NCBI Accession: NP_001190991

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy