STXBP1 antibody (pSer515)

Catalog No. ABIN318802

This Rabbit Polyclonal antibody specifically detects STXBP1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Monkey, Dog, Chicken and Xenopus laevis.

Quick Overview for STXBP1 antibody (pSer515) (ABIN318802)

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Dog, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-STXBP1 Antibody

Binding Specificity: pSer515

Specificity: This antibody recognises Munc-18 phosphorylated at serine 515.

Purification: Purified

Immunogen: Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated Ser515 of Munc-18.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 1000, detects a band/s of approximately 65 kDa in rat cortex cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM Hepes pH 7.5 containing 0.09 % Sodium Azide (NaN3), 50 % Glycerol and 0.01 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STXBP1

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Alternative Name: STXBP1 / UNC18A

Background: Munc-18 is a 67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter release, facilitating synaptic vesicle docking and fusion. It inhibits the formation of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex by binding syntaxin 1 preventing the other components (synaptobrevin and synaptosomal associated protein of 25 kDa (SNAP-25)) from binding. The function of Munc-18 is regulated by the phosphorylation of serine 515 by protein kinase C. Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release.Synonyms: N-Sec1, Syntaxin-binding protein 1, Unc-18 homolog, Unc-18-1, Unc-18A

Gene ID: 25558

NCBI Accession: NP_037170

UniProt: P61765

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport