MAP2K5 antibody (pSer311, pThr315)

Catalog No. ABIN318850

This Rabbit Polyclonal antibody specifically detects MAP2K5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Chicken, Monkey, Xenopus laevis and Zebrafish (Danio rerio).

Quick Overview for MAP2K5 antibody (pSer311, pThr315) (ABIN318850)

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Monkey, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP2K5 Antibody

Binding Specificity: pSer311, pThr315

Specificity: This antibody specifically recognises 49 kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.

Purification: Purified

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes phoshorylated Ser311 and Thr315.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 1000, detects a band of approximately 49 kDa in rat testes cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM Hepes pH 7.5 containing 0.09 % Sodium Azide (NaN3), 0.01 % Bovine Serum Albumin, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MAP2K5

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Alternative Name: MAP2K5

Background: MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation.Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5

Gene ID: 5607

NCBI Accession: NP_001193733

UniProt: Q13163

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway