PDCD4 antibody (C-Term)

Catalog No. ABIN318876

This anti-PDCD4 antibody is a Rabbit Polyclonal antibody detecting PDCD4 in WB and EIA. Suitable for Human, Mouse, Rat and Xenopus laevis.

Quick Overview for PDCD4 antibody (C-Term) (ABIN318876)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PDCD4 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects a specific epitope within the carboxy-terminal of programmed cell death 4 (Pdcd4), a novel tumour suppressor.

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids near the carboxy terminal of human Pdcd4.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 1: 100 - 1: 300, requires protein digestionpretreatment of paraffin sections e. g. trypsin or pronase. ELISA: 1: 10000 - 1: 50000. Western Blot: 1: 1000 - 1: 10000, detects a band of approximately 52 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.01 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store at +4 °C or at -20 °C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4

Background: Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis. Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility. Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells. Pdcd4 expression is down regulated with tumour progression in carcinomas of the lung, colon, prostate and breast.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gene ID: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6