DDC antibody

Catalog No. ABIN318954

This anti-DDC antibody is a Rabbit Polyclonal antibody detecting DDC in WB. Suitable for Rat and Cow.

Quick Overview for DDC antibody (ABIN318954)

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reactivity: Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDC Antibody

Specificity: This antibody recognises DOPA decarboxylase (aromatic-L-amino acid decarboxylase, DDC).

Purification: Affinity Chromatography.

Immunogen: SDS denatured, recombinant bovine DOPA decarboxylase expressed E. coli and purified from inclusion bodies.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000. This antibody detects a band of approximately 55 kDa in rat adrenalmedulla.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES pH 7.5, 150 mM NaCl containing 0.09 % Sodium Azide as preservative and 0.01 % BSA, 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DDC

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Alternative Name: AADC / DDC

Background: DOPA decarboxylase is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Following the hydroxylation of tyrosine to form L dihydroxyphenylalanine (LDOPA), catalyzed by tyrosine hydroxylase, DDC decarboxylates LDOPA to form dopamine. This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia. Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla. In the nervous system, tryptophan hydroxylase produces 5 OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin. DDC is a homodimeric, pyridoxal phosphate dependent enzyme.Synonyms: Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase

Gene ID: 280762

NCBI Accession: NP_776332

UniProt: P27718

Pathways: Dopaminergic Neurogenesis