Dynamin 1 antibody (pSer774)

Catalog No. ABIN319098

This anti-Dynamin 1 antibody is a Sheep Polyclonal antibody detecting Dynamin 1 in WB, IF and IHC (fro). Suitable for Human, Mouse, Rat, Dog, Cow, Chicken and Monkey.

Quick Overview for Dynamin 1 antibody (pSer774) (ABIN319098)

Target: Dynamin 1 (DNM1)

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Monkey

Host: Sheep

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Dynamin 1 Antibody

Binding Specificity: pSer774

Specificity: This antibody is specific for Dynamin when phosphorylated at Serine 774.

Cross-Reactivity (Details): Species reactivity (expected):Human, Monkey, Bovine, Chicken, Mouse, Dog.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within Dynamin which includes phosphorylated Ser774. Genename: DNM

Isotype: IgG

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on Frozen Sections. Western Blot (1/1000): This antibody detects a band of approximately 95 kDa in Rathippocampus lysates. For the detection of Phosphoproteins, Serine Phosphatase inhibitors such as 10 mMSodium Fluoride should be added to the sample buffer. Milk or other casein-basedblocking solutions are not recommended as casein is a Phosphoprotein and its use canresult in high background.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % BSA and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin-1

Background: Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.Synonyms: DNM, DNM1

Gene ID: 140694

NCBI Accession: NP_542420

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling