Tryptophan Hydroxylase 1 antibody

Catalog No. ABIN319127

The Sheep Polyclonal anti-Tryptophan Hydroxylase 1 antibody has been validated for WB and IHC (fro). It is suitable to detect Tryptophan Hydroxylase 1 in samples from Human and Rat.

Quick Overview for Tryptophan Hydroxylase 1 antibody (ABIN319127)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Human, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tryptophan Hydroxylase 1 Antibody

Specificity: This antibody recognises Tryptophan Hydroxylase (TPH) which catalyses the 5-hydroxylation of tryptophan, in the first stage of indoleamine biosynthesis. Serotonin biosynthesis occurs principally in neurons which originate in the raphe nucleus of the brain, and melatonin synthesis takes place within the pineal gland.

Purification: Affinity chromatography

Immunogen: Recombinant rabbit Tryptophan Hydroxylase purified from E. coli.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1: 1000. Western Blot: 1: 1000, detects a band of approximately 55 kDa in human dorsal Raphenucleus lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % Bovine Serum Albumin and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: Tryptophan 5-Hydroxylase 1 (TPH1)

Background: Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1

Gene ID: 7166

NCBI Accession: NP_004170

UniProt: P17752

Pathways: Feeding Behaviour